Journal: Advanced Science

Article Title: The Characteristics of Tumor Microenvironment by the Single Cell Profile in Endometrial Cancer of no Specific Molecular Profile (NSMP) Subtype

doi: 10.1002/advs.78332

Figure Lengend Snippet: Macrophages with highly expressing SPP1 exhibit enhanced interactions with CD4+ T cells and epithelial cells expressing CD44 in R NSMP EC. (A) Chord diagram illustrates the SPP1 signaling in the R tumors. (B) Circle plots demonstrate the cell‐cell communications among Epi_C2, Epi_C3, macrophages, and CD4+ T cells. (C) The ligand‐receptor pair analysis among Epi_C2, Epi_C3, SPP1+ TAMs, and MKI67+ TAMs. SPP1‐CD44 axis emerged as the primary contributor to this interaction. (D) Representative images of mIHC staining for the SPP1+ CD68+ TAMs and CD44+ CD4+ T cells in NR and R NSMP EC. (E) The histogram illustrates the fraction of SPP1+ CD68+ TAMs in NR and R tumors. Data presented as mean ± SD. The p ‐value was calculated by Student's t‐test. (F) The spatial dot plots show the co‐localization of CD44 and SPP1 in NR and R tumors. Dotted lines indicate the boundary of the tumor. (G) The spatial position of SPP1+ CD68+ macrophages and CD44+ epithelial cells. White arrows indicate SPP1+ CD68+ macrophages; Orange arrows indicate SPP1‐ CD68+ macrophages. * p < 0.05.

Article Snippet: The primary antibodies were: EPCAM (21050‐1‐AP, Proteintech), CD68 (25747‐1‐AP, Proteintech), SPP1 (22952‐1‐AP, Proteintech), CD4 (67786‐1‐lg, Proteintech) and CD44 (15675‐1‐AP, Proteintech).

Techniques: Expressing, Analysis, Staining

Journal: Advanced Science

Article Title: The Characteristics of Tumor Microenvironment by the Single Cell Profile in Endometrial Cancer of no Specific Molecular Profile (NSMP) Subtype

doi: 10.1002/advs.78332

Figure Lengend Snippet: SPP1+ macrophages promote T cell stress and maintain epithelial cell stemness. (A, B) Western blot and RT‐qPCR results of SPP1 expression in macrophages with SPP1 knockdown. (C, D) Western blot and RT‐qPCR results of SPP1 expression in macrophages with overexpression of SPP1. (E) Western blot analysis of the protein expression of HSP70, LAG3, and PD‐1 in Jurkat cells after co‐culture with control and SPP1 knockdown macrophages. (F) qRT‐PCR analysis of the transcriptional level of HSPA1A and HSPA1B in Jurkat cells after co‐culture with control and SPP1 knockdown macrophages. (G) Western blot analysis of the protein expression of HSP70, LAG3, and PD‐1 in Jurkat cells after co‐culture with control, SPP1 overexpression, and SPP1 overexpression plus anti‐CD44 macrophages. (H) qRT‐PCR analysis of the transcriptional level of HSPA1A and HSPA1B in Jurkat cells after co‐culture with control, SPP1 overexpression, and SPP1 overexpression plus anti‐CD44 macrophages. (I) Western blot analysis of the protein expression of CD44 and CD133 in Ishikawa cells after co‐culture with control and SPP1 knockdown macrophages. (J) Western blot analysis of the protein expression of CD44 and CD133 in Ishikawa cells after co‐culture with control, SPP1 overexpression, and SPP1 overexpression plus anti‐CD44 macrophages. (K) Sphere formation assay was used to detect the stemness ability of Ishikawa cells after co‐culture with control and SPP1 knockdown macrophages. (L) A sphere formation assay was used to detect the stemness ability of Ishikawa cells after co‐culture with control, SPP1 overexpression, and SPP1 overexpression plus anti‐CD44 macrophages. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not significant.

Article Snippet: The primary antibodies were: EPCAM (21050‐1‐AP, Proteintech), CD68 (25747‐1‐AP, Proteintech), SPP1 (22952‐1‐AP, Proteintech), CD4 (67786‐1‐lg, Proteintech) and CD44 (15675‐1‐AP, Proteintech).

Techniques: Western Blot, Quantitative RT-PCR, Expressing, Knockdown, Over Expression, Analysis, Co-culture Assay, Control, Tube Formation Assay

Journal: iScience

Article Title: TXNRD1-driven enteric glial remodeling promotes neuronal dysfunction in Hirschsprung disease

doi: 10.1016/j.isci.2026.117636

Figure Lengend Snippet:

Article Snippet: Rabbit anti-SPP1 , Proteintech , Cat# 22952-1-AP; RRID: AB_2783651.

Techniques: Model Organisms, Recombinant, CCK-8 Assay, Apoptosis Assay, ROS Assay, Calcium Assay, Enzyme-linked Immunosorbent Assay, Generated, Software, Genotyping

Journal: NPJ Precision Oncology

Article Title: Multi-omics profiling reveals tumor microenvironment characteristics linked to immunotherapy response and prognosis in non-small cell lung cancer

doi: 10.1038/s41698-026-01474-2

Figure Lengend Snippet: A Identification of four celltypes in the LLC TME by scRNA-seq. B Anti-SPP1 therapy increases all immune subsets, decreases non-immune cells. C Subclustering of T/NK cells. D GO enrichment analysis of genes highly expressed in CD8 + T cells at different groups (control vs. anti-SPP1). E Anti-SPP1 therapy decreases M2-like macrophage proportion. F Immunofluorescence indicated increased CD8 + T infiltration with concurrent M2 reduction post-treatment.

Article Snippet: Five days post-inoculation, mice with palpable tumors were randomly assigned to four treatment groups ( n = 4 per group) using a random number table : Control (PBS) , Anti-PD-1 (clone RMP1-14, Bio X Cell, #BE0146) , Anti-SPP1 (anti-mouse osteopontin antibody, clone 103D6, MCE), and Anti-SPP1 combined with Anti-PD-1.

Techniques: Control, Immunofluorescence